Total retrieval time of time of 45 minutes at 75-80 degrees celcius for nuclear antigen such as ki-67, ER, PR , etc. A must for cell line preps and delicate tissues such as brain , bone marrow and animal tissues.
Antigen retrieval is a non-enzymatic pretreatment for immunostaining to reduce or eliminate the chemical modifications caused by formalin fixation, through high temperature heating or strong alkaline solution (non-heating).
Antigen retrieval is one of the most critical steps, and getting it right is the key to unlocking clear, reproducible data. In this guide, we'll share our expertise on why it matters and how to perform it correctly.
